Description: Synthetic miRNA Agomir negative control for experiments using targeted miRNA transfection Form: Lyophilized powder Directions for Use : Briefly centrifuge tubes containing miRNA agomir to ensure that the miRNA pellet is located at the bottom of the tube. Dissolve miRNA agomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water). For example: dissolve 5 nmol miRNA agomir to 20 µM using 250 µl DEPC H2O (or RNase-free water). Pipette the solution up and down 3-5 times (or vortex briefly). Briefly centrifuge tubes containing miRNA agomir to ensure that the solution is collected at the bottom of the tube. Aliquot the miRNA agomir into small volumes and store at ≤ -20°C. miRNA agomir is stable (for 6 months under the specified storage condition). For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times.Components : This synthetic miRNA is based on the mature miRNA sequence. It does not contain the full precursor miRNA stem-loop.Storage/Stability : Shipped at 4 °C. Store at -20 °C for one year. Avoid freeze-thaw cycles after reconstitution.