Goat Anti-Rabbit IgG (H&L) - AcalephFluor790-Secondary Antibody-Cohesion Biosciences

Line colors represent the approximate visible colors of the wavelength maxima.
Western blot analysis of Beta-actin expression in Jurkat (A), MCF7 (B), NIH3T3 (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates. Antibody binding was detected using Goat Anti-Rabbit IgG (H&L) - AF790 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

Product Name	Goat Anti-Rabbit IgG (H&L) - AcalephFluor790
Cat No:	CSA4211
Source:	Goat
Reactivity:	Rb
Applications:	E, WB, IH, IF, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

100 μl

$20

500 μl

$60

1 ml

$90

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Description: Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) - AcalephFluor790

Immunogen: Rabbit IgG

Purification: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Clonality: Polyclonal

Conjugation: AcalephFluor790

Form: Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

Dilution: WB (1/5000 - 1/20000), IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Platform : Ex/Em = 770/794 nm

Line colors represent the approximate visible colors of the wavelength maxima.
Western blot analysis of Beta-actin expression in Jurkat (A), MCF7 (B), NIH3T3 (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates. Antibody binding was detected using Goat Anti-Rabbit IgG (H&L) - AF790 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

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