Description: Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) HRP polymer labled Immunogen: Mouse IgG Purification: Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse, rabbit and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Clonality: Polyclonal Conjugation: HRP polymer Form: 0.5 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.05% Proclin Dilution: E (1/5000 - 1/20000), WB (1/5000 - 1/20000), IH (1/100 - 1/500), IC (1/100 - 1/500) Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Immunohistochemical analysis staining in human liver carcinoma formalin fixed paraffin-embedded tissue section. The section was pre-treated using pressure cooker heat antigen retrieval with sodium citrate buffer (0.01M, pH=6) for 3 minutes. The section was detected using mouse primary antibody, and Goat Anti-Mouse IgG (H&L)-HRP polymer. The section was then counterstained with haematoxylin and mounted with Neutral Gum.