Description: Recombinant Rat IL-12 Protein is produced by mammalian expression system and the target gene encoding Met23-Ser335 (p40) & Arg23-Ser215 (p35) is expressed with a 6His tag at the C-terminus. Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. Gene Symbol: IL12A; IL12B Alternative Names: NKSF1; NKSF2; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2Entrez Gene (Rat) : 84405; 64546SwissProt (Rat) : Q9R278; Q9R103Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MWELEKDVYV VEVDWRPDAP GETVTLTCDS PEEDDITWTS DQRRGVIGSG KTLTITVREF LDAGQYTCHR GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VHRNTDLKFN IKSSSSSPES RAVTCGRASL SAEKVTLNQR DYEKYSVACQ EDVTCPTAEE TLPIELVVEA QQQNKYENYS TSFFIRDIIK PDPPKNLQVK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKTK ETEEECNQKG AFLVEKTSAE VQCKGANICV QAQDRYYNSS CSKWTCVPCR GRSHHHHHH& RVIPVSGPAK CLNQSQNLLK TTDDMVRTAR EKLKHYSCTA GDIDHEDITR DKTSTLEACL PLELHKNESC LATKETSSII RGSCLPPQKT SLMMTLCLGS IYEDLKMYQS EFQAINAALQ SHNHQQITLD RNMLMAIDEL MRSLNHSGET LHQKAPMGEA DPYRVKMKLC ILLHAFSTRV MTINRVMNYL SSSHHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Quality Control : Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.