Description: Recombinant Mouse SHH Protein is produced by E.coli expression system and the target gene encoding Cys25-Gly198 (Cys25Ile-Ile) is expressed. Form: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4. Gene Symbol: SHH Alternative Names: Sonic hedgehog protein; SHH; HHG-1Entrez Gene (Mouse) : 20423SwissProt (Mouse) : Q62226Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Quality Control : Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.