Description: Recombinant Human Semaphorin 3A Protein is produced by mammalian expression system and the target gene encoding Lys26-Phe546 is expressed with a 6His-Flag tag at the N-terminus. Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. Gene Symbol: SEMA3A Alternative Names: SEMAD; Semaphorin-3A; Semaphorin III; Sema IIIEntrez Gene (Human): 10371SwissProt (Human): Q14563Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : GSGSHHHHHH DYKDDDDKKN NVPRLKLSYK EMLESNNVIT FNGLANSSSY HTFLLDEERS RLYVGAKDHI FSFDLVNIKD FQKIVWPVSY TRRDECKWAG KDILKECANF IKVLKAYNQT HLYACGTGAF HPICTYIEIG HHPEDNIFKL ENSHFENGRG KSPYDPKLLT ASLLIDGELY SGTAADFMGR DFAIFRTLGH HHPIRTEQHD SRWLNDPKFI SAHLISESDN PEDDKVYFFF RENAIDGEHS GKATHARIGQ ICKNDFGGHR SLVNKWTTFL KARLICSVPG PNGIDTHFDE LQDVFLMNFK DPKNPVVYGV FTTSSNIFKG SAVCMYSMSD VRRVFLGPYA HRDGPNYQWV PYQGRVPYPR PGTCPSKTFG GFDSTKDLPD DVITFARSHP AMYNPVFPMN NRPIVIKTDV NYQFTQIVVD RVDAEDGQYD VMFIGTDVGT VLKVVSIPKE TWYDLEEVLL EEMTVFREPT AISAMELSTK QQQLYIGSTA GVAQLPLHRC DIYGKACAEC CLARDPYCAW DGSACSRYFDirections for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.