Description: Recombinant Human Factor X Protein is produced by mammalian expression system and the target gene encoding Asn32-Lys488 is expressed with a Fc tag at the C-terminus. Form: Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 0.2mM CaCl2, pH5.5. Gene Symbol: F10 Alternative Names: Coagulation factor X; Stuart factor; Stuart-Prower factorEntrez Gene (Human): 2159SwissProt (Human): P00742Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : NNILARVTRA NSFLEEMKKG HLERECMEET CSYEEAREVF EDSDKTNEFW NKYKDGDQCE TSPCQNQGKC KDGLGEYTCT CLEGFEGKNC ELFTRKLCSL DNGDCDQFCH EEQNSVVCSC ARGYTLADNG KACIPTGPYP CGKQTLERRK RSVAQATSSS GEAPDSITWK PYDAADLDPT ENPFDLLDFN QTQPERGDNN LTRIVGGQEC KDGECPWQAL LINEENEGFC GGTILSEFYI LTAAHCLYQA KRFKVRVGDR NTEQEEGGEA VHEVEVVIKH NRFTKETYDF DIAVLRLKTP ITFRMNVAPA CLPERDWAES TLMTQKTGIV SGFGRTHEKG RQSTRLKMLE VPYVDRNSCK LSSSFIITQN MFCAGYDTKQ EDACQGDSGG PHVTRFKDTY FVTGIVSWGE GCARKGKYGI YTKVTAFLKW IDRSMKTRGL PKAKSHAPEV ITSSPLKDIE GRMDEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Quality Control : Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.