Description: Recombinant Human ECE1 Protein is produced by mammalian expression system and the target gene encoding Gln90-Trp770 is expressed with a 8His tag at the N-terminus. Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. Gene Symbol: ECE1 Alternative Names: Endothelin-converting enzyme 1; ECE-1Entrez Gene (Human): 1889SwissProt (Human): P42892Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : HHHHHHHHQY QTRSPSVCLS EACVSVTSSI LSSMDPTVDP CHDFFSYACG GWIKANPVPD GHSRWGTFSN LWEHNQAIIK HLLENSTASV SEAERKAQVY YRACMNETRI EELRAKPLME LIERLGGWNI TGPWAKDNFQ DTLQVVTAHY RTSPFFSVYV SADSKNSNSN VIQVDQSGLG LPSRDYYLNK TENEKVLTGY LNYMVQLGKL LGGGDEEAIR PQMQQILDFE TALANITIPQ EKRRDEELIY HKVTAAELQT LAPAINWLPF LNTIFYPVEI NESEPIVVYD KEYLEQISTL INTTDRCLLN NYMIWNLVRK TSSFLDQRFQ DADEKFMEVM YGTKKTCLPR WKFCVSDTEN NLGFALGPMF VKATFAEDSK SIATEIILEI KKAFEESLST LKWMDEETRK SAKEKADAIY NMIGYPNFIM DPKELDKVFN DYTAVPDLYF ENAMRFFNFS WRVTADQLRK APNRDQWSMT PPMVNAYYSP TKNEIVFPAG ILQAPFYTRS SPKALNFGGI GVVVGHELTH AFDDQGREYD KDGNLRPWWK NSSVEAFKRQ TECMVEQYSN YSVNGEPVNG RHTLGENIAD NGGLKAAYRA YQNWVKKNGA EHSLPTLGLT NNQLFFLGFA QVWCSVRTPE SSHEGLITDP HSPSRFRVIG SLSNSKEFSE HFRCPPGSPM NPPHKCEVW Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Quality Control : Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.