Description: Recombinant Human WIF1 Protein is produced by Mammalian expression system and the target gene encoding Gly29-Trp379 is expressed with a 6His tag at the C-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.5% CHAPS, pH 4.0. Gene Symbol: WIF1 Alternative Names: Wnt inhibitory factor 1; WIF-1Entrez Gene (Human): 11197SwissProt (Human): Q9Y5W5Purity : Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure : GPPQEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMP AIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVA AFEVDVIVMN SEGNTILKTP QNAIFFKTCQ QAECPGGCRN GGFCNERRIC ECPDGFHGPH CEKALCTPRC MNGGLCVTPG FCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCEISKC PQPCRNGGKC IGKSKCKCSK GYQGDLCSKP VCEPGCGAHG TCHEPNKCQC QEGWHGRHCN KRYEASLIHA LRPAGAQLRQ HTPSLKKAEE RRDPPESNYI WVDHHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.