Description: Recombinant Human VCL Protein is produced by E. coli expression system and the target gene encoding Pro2-Gln1066 is expressed. Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. Gene Symbol: VCL Alternative Names: Vinculin; Metavinculin; MVEntrez Gene (Human): 7414SwissProt (Human): P18206Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : PVFHTRTIES ILEPVAQQIS HLVIMHEEGE VDGKAIPDLT APVAAVQAAV SNLVRVGKET VQTTEDQILK RDMPPAFIKV ENACTKLVQA AQMLQSDPYS VPARDYLIDG SRGILSGTSD LLLTFDEAEV RKIIRVCKGI LEYLTVAEVV ETMEDLVTYT KNLGPGMTKM AKMIDERQQE LTHQEHRVML VNSMNTVKEL LPVLISAMKI FVTTKNSKNQ GIEEALKNRN FTLEKMSAEI NEIIRVLQLT SWDEDAWASK DTEAMKRALA SIDSKLNQAK GWLRDPSASP GDAGEQAIRQ ILDEAGKVGE LCAGKERREI LGTCKMLGQM TDQVADLRAR GQGSSPVAMQ KAQQVSQGLD VLTAKVENAA RKLEAMTNSK QSIAKKIDAA QNWLADPNGG PEGEEQIRGA LAEARKIAEL CDDPKERDDI LRSLGEISAL TSKLADLRRQ GKGDSPEARA LAKQVATALQ NLQTKTNRAV ANSRPAKAAV HLEGKIEQAQ RWIDNPTVDD RGVGQAAIRG LVAEGHRLAN VMMGPYRQDL LAKCDRVDQL TAQLADLAAR GEGESPQARA LASQLQDSLK DLKARMQEAM TQEVSDVFSD TTTPIKLLAV AATAPPDAPN REEVFDERAA NFENHSGKLG ATAEKAAAVG TANKSTVEGI QASVKTAREL TPQVVSAARI LLRNPGNQAA YEHFETMKNQ WIDNVEKMTG LVDEAIDTKS LLDASEEAIK KDLDKCKVAM ANIQPQMLVA GATSIARRAN RILLVAKREV ENSEDPKFRE AVKAASDELS KTISPMVMDA KAVAGNISDP GLQKSFLDSG YRILGAVAKV REAFQPQEPD FPPPPPDLEQ LRLTDELAPP KPPLPEGEVP PPRPPPPEEK DEEFPEQKAG EVINQPMMMA ARQLHDEARK WSSKGNDIIA AAKRMALLMA EMSRLVRGGS GTKRALIQCA KDIAKASDEV TRLAKEVAKQ CTDKRIRTNL LQVCERIPTI STQLKILSTV KATMLGRTNI SDEESEQATE MLVHNAQNLM QSVKETVREA EAASIKIRTD AGFTLRWVRK TPWYQ Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.