Description: Recombinant Human TPK1 Protein is produced by mammalian expression system and the target gene encoding Met1-Ser243 is expressed with a 6His tag at the C-terminus. Form: Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Gene Symbol: TPK1 Alternative Names: Thiamin pyrophosphokinase 1; hTPK1; Placental protein 20; PP20; Thiamine pyrophosphokinase 1Entrez Gene (Human): 27010SwissProt (Human): Q9H3S4Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MEHAFTPLEP LLSTGNLKYC LVILNQPLDN YFRHLWNKAL LRACADGGAN RLYDITEGER ESFLPEFING DFDSIRPEVR EYYATKGCEL ISTPDQDHTD FTKCLKMLQK KIEEKDLKVD VIVTLGGLAG RFDQIMASVN TLFQATHITP FPIIIIQEES LIYLLQPGKH RLHVDTGMEG DWCGLIPVGQ PCSQVTTTGL KWNLTNDVLA FGTLVSTSNT YDGSGVVTVE TDHPLLWTMA IKSVDHHHHH H Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.