Description: Recombinant Rabbit TNF Protein is produced by E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17.4 Daltons. Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 300mM NaCl, pH 7.4. Gene Symbol: TNF Alternative Names: TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-aEntrez Gene (Human): 100009088 (Rabbit)SwissProt (Human): P04924 (Rabbit)Purity : Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure : MLRSASRALS DKPLAHVVAN PQVEGQLQWL SQRANALLAN GMKLTDNQLV VPADGLYLIY SQVLFSGQGC RSYVLLTHTV SRFAVSYPNK VNLLSAIKSP CHRETPEEAE PMAWYEPIYL GGVFQLEKGD RLSTEVNQPE YLDLAESGQV YFGIIAL Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.