Description: Recombinant Human THPO Protein is produced by mammalian expression system and the target gene encoding Ser22-Gly353 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. Gene Symbol: THPO Alternative Names: MGDF; Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligandEntrez Gene (Human): 7066SwissProt (Human): P40225Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : HHHHHHSPAP PACDLRVLSK LLRDSHVLHS RLSQCPEVHP LPTPVLLPAV DFSLGEWKTQ MEETKAQDIL GAVTLLLEGV MAARGQLGPT CLSSLLGQLS GQVRLLLGAL QSLLGTQLPP QGRTTAHKDP NAIFLSFQHL LRGKVRFLML VGGSTLCVRR APPTTAVPSR TSLVLTLNEL PNRTSGLLET NFTASARTTG SGLLKWQQGF RAKIPGLLNQ TSRSLDQIPG YLNRIHELLN GTRGLFPGPS RRTLGAPDIS SGTSDTGSLP PNLQPGYSPS PTHPPTGQYT LFPLPPTLPT PVVQLHPLLP DPSAPTPTPT SPLLNTSYTH SQNLSQEGVD HHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.