Description: Recombinant Human SMAD1 Protein is produced by E. coli expression system and the target gene encoding Met1-Ser465 is expressed with a GST tag at the N-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. Gene Symbol: SMAD1 Alternative Names: BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1Entrez Gene (Human): 4086SwissProt (Human): Q15797Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMNV TSLFSFTSPA VKRLLGWKQG DEEEKWAEKA VDALVKKLKK KKGAMEELEK ALSCPGQPSN CVTIPRSLDG RLQVSHRKGL PHVIYCRVWR WPDLQSHHEL KPLECCEFPF GSKQKEVCIN PYHYKRVESP VLPPVLVPRH SEYNPQHSLL AQFRNLGQNE PHMPLNATFP DSFQQPNSHP FPHSPNSSYP NSPGSSSSTY PHSPTSSDPG SPFQMPADTP PPAYLPPEDP MTQDGSQPMD TNMMAPPLPS EINRGDVQAV AYEEPKHWCS IVYYELNNRV GEAFHASSTS VLVDGFTDPS NNKNRFCLGL LSNVNRNSTI ENTRRHIGKG VHLYYVGGEV YAECLSDSSI FVQSRNCNYH HGFHPTTVCK IPSGCSLKIF NNQEFAQLLA QSVNHGFETV YELTKMCTIR MSFVKGWGAE YHRQDVTSTP CWIEIHLHGP LQWLDKVLTQ MGSPHNPISS VS Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.