Description: Recombinant Mouse SHH Protein is produced by E. coli expression system and the target gene encoding Cys25-Gly198 is expressed. Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4. Gene Symbol: SHH Alternative Names: Sonic hedgehog protein; SHH; HHG-1Entrez Gene (Mouse) : 20423SwissProt (Mouse) : Q62226Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGG Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.