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Recombinant Human SERPINA12 Protein CRP2272
Product NameRecombinant Human SERPINA12 Protein
Cat No: CRP2272
Source: E. coli
Reactivity: Human
Applications: E, WB, SDS-PAGE, MS
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
10 μg
$140
50 μg
$420
500 μg
$1680
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Description: Recombinant Human SERPINA12 Protein is produced by E.coli expression system and the target gene encoding Leu21-Lys414 is expressed with a GST tag at the N-terminus.
Form: Liquid in a 0.2 μM filtered solution of 50mM Tris-HCl, 160mM NaCl, 0.2mM PMSF, 1mM DTT, 10% Glycerine, pH 7.2.
Gene Symbol: SERPINA12
Alternative Names: Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin
Entrez Gene (Human): 145264
SwissProt (Human): Q8IW75
Purity : Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Chemical Structure : MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSLKPS FSPRNYKALS EVQGWKQRMA AKELARQNMD LGFKLLKKLA FYNPGRNIFL SPLSISTAFS MLCLGAQDST LDEIKQGFNF RKMPEKDLHE GFHYIIHELT QKTQDLKLSI GNTLFIDQRL QPQRKFLEDA KNFYSAETIL TNFQNLEMAQ KQINDFISQK THGKINNLIE NIDPGTVMLL ANYIFFRARW KHEFDPNVTK EEDFFLEKNS SVKVPMMFRS GIYQVGYDDK LSCTILEIPY QKNITAIFIL PDEGKLKHLE KGLQVDTFSR WKTLLSRRVV DVSVPRLHMT GTFDLKKTLS YIGVSKIFEE HGDLTKIAPH RSLKVGEAVH KAELKMDERG TEGAAGTGAQ TLPMETPLVV KIDKPYLLLI YSEKIPSVLF LGKIVNPIGK
Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability : Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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