Description: Recombinant Human SEMA4D Protein is produced by mammalian expression system and the target gene encoding Met22-Arg734 is expressed with a 6His tag at the C-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Gene Symbol: SEMA4D Alternative Names: C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; GR3; CD100Entrez Gene (Human): 10507SwissProt (Human): Q92854Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MAFAPIPRIT WEHREVHLVQ FHEPDIYNYS ALLLSEDKDT LYIGAREAVF AVNALNISEK QHEVYWKVSE DKKAKCAEKG KSKQTECLNY IRVLQPLSAT SLYVCGTNAF QPACDHLNLT SFKFLGKNED GKGRCPFDPA HSYTSVMVDG ELYSGTSYNF LGSEPIISRN SSHSPLRTEY AIPWLNEPSF VFADVIRKSP DSPDGEDDRV YFFFTEVSVE YEFVFRVLIP RIARVCKGDQ GGLRTLQKKW TSFLKARLIC SRPDSGLVFN VLRDVFVLRS PGLKVPVFYA LFTPQLNNVG LSAVCAYNLS TAEEVFSHGK YMQSTTVEQS HTKWVRYNGP VPKPRPGACI DSEARAANYT SSLNLPDKTL QFVKDHPLMD DSVTPIDNRP RLIKKDVNYT QIVVDRTQAL DGTVYDVMFV STDRGALHKA ISLEHAVHII EETQLFQDFE PVQTLLLSSK KGNRFVYAGS NSGVVQAPLA FCGKHGTCED CVLARDPYCA WSPPTATCVA LHQTESPSRG LIQEMSGDAS VCPDKSKGSY RQHFFKHGGT AELKCSQKSN LARVFWKFQN DVLKAESPKY GLMGRKNLLI FNLSEGDSGV YQCLSEERVK NKTVFQVVAK HVLEVKVVPK PVVAPTLSVV QTEGSRIATK VLVASTQGSS PPTPAVQATS SGAITLPPKP APTGTSCEPK IVINTVPQLH SEKTMYLKSS DNRVDHHHHH H Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.