Description: Recombinant Mouse SEMA4A Protein is produced by mammalian expression system and the target gene encoding Thr33-His682 is expressed with a 6His tag at the C-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Gene Symbol: SEMA4A Alternative Names: SEMAB; SEMB; Semaphorin-4A; Semaphorin-B; Sema BEntrez Gene (Mouse) : 20351SwissProt (Mouse) : Q62178Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : TGGQGPMPRV KYHAGDGHRA LSFFQQKGLR DFDTLLLSDD GNTLYVGARE AVLALNIQNP GIPRLKNMIP WPASERKKTE CAFKKKSNET QCFNFIRVLV SYNATHLYAC GTFAFSPACT FIELQDSLLL PILIDKVMDG KGQSPFDPVH KHTAVLVDGM LYSGTMNNFL GSEPILMRTL GSQPVLKTDI FLRWLHADAS FVAAIPSTQV VYFFFEETAS EFDFFEELYI SRVAQVCKND VGGEKLLQKK WTTFLKAQLL CAQPGQLPFN IIRHAVLLPA DSPSVSRIYA VFTSQWQVGG TRSSAVCAFS LTDIERVFKG KYKELNKETS RWTTYRGSEV SPRPGSCSMG PSSDKALTFM KDHFLMDEHV VGTPLLVKSG VEYTRLAVES ARGLDGSSHV VMYLGTSTGS LHKAVVPQDS SAYLVEEIQL SPDSEPVRNL QLAPAQGAVF AGFSGGIWRV PRANCSVYES CVDCVLARDP HCAWDPESRL CSLLSGSTKP WKQDMERGNP EWVCTRGPMA RSPRRQSPPQ LIKEVLTVPN SILELPCPHL SALASYHWSH GRAKISEASA TVYNGSLLLL PQDGVGGLYQ CVATENGYSY PVVSYWVDSQ DQPLALDPEL AGVPRERVQV PLTRVGGGAS MAAQRSYWPH VDHHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.