Description: Recombinant Human RSPO1 Protein is produced by mammalian expression system and the target gene encoding Arg31-Ala263 is expressed with a 6His tag at the C-terminus. Form: Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. Gene Symbol: RSPO1 Alternative Names: R-spondin-1; Roof plate-specific spondin-1; hRspo1Entrez Gene (Human): 284654SwissProt (Human): Q2MKA7Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPAVDHHHHH H Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.