Description: Recombinant Human POMGNT1 Protein is produced by Mammalian expression system and the target gene encoding Leu59-Thr660 is expressed with a 6His tag at the C-terminus. Form: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5. Gene Symbol: POMGNT1 Alternative Names: MGAT1.2; Protein O-linked-mannose beta-12-N-acetylglucosaminyltransferase 1; POMGnT1; UDP-GlcNAc:alpha-D-mannoside beta-12-N-acetylglucosaminyltransferase I.2; GnT I.2Entrez Gene (Human): 55624SwissProt (Human): Q8WZA1Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : LDTRRAISEA NEDPEPEQDY DEALGRLEPP RRRGSGPRRV LDVEVYSSRS KVYVAVDGTT VLEDEAREQG RGIHVIVLNQ ATGHVMAKRV FDTYSPHEDE AMVLFLNMVA PGRVLICTVK DEGSFHLKDT AKALLRSLGS QAGPALGWRD TWAFVGRKGG PVFGEKHSKS PALSSWGDPV LLKTDVPLSS AVEAECHWAD TELNRRRRRF CSKVEGYGSV CSCKDPTPIE FSPDPLPDNK VLNVPVAVIA GNRPNYLYRM LRSLLSAQGV SPQMITVFID GYYEEPMDVV ALFGLRGIQH TPISIKNARV SQHYKASLTA TFNLFPEAKF AVVLEEDLDI AVDFFSFLSQ SIHLLEEDDS LYCISAWNDQ GYEHTAEDPA LLYRVETMPG LGWVLRRSLY KEELEPKWPT PEKLWDWDMW MRMPEQRRGR ECIIPDVSRS YHFGIVGLNM NGYFHEAYFK KHKFNTVPGV QLRNVDSLKK EAYEVEVHRL LSEAEVLDHS KNPCEDSFLP DTEGHTYVAF IRMEKDDDFT TWTQLAKCLH IWDLDVRGNH RGLWRLFRKK NHFLVVGVPA SPYSVKKPPS VTPIFLEPPP KEEGAPGAPE QTVDHHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.