Description: Recombinant E. coli NAGK Protein is produced by E. coli expression system and the target gene encoding Met1-Asp303 is expressed with a 6His tag at the C-terminus. Form: Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 2mM DTT, 20% Glycerol, pH 8.0. Gene Symbol: NAGK Alternative Names: ycfX; N-acetyl-D-glucosamine kinase; GlcNAc kinaseEntrez Gene (Human): 945664 (E. coli)SwissProt (Human): P75959 (E. coli)Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MNHKVHHHHH HMYYGFDIGG TKIALGVFDS GRQLQWEKRV PTPRDSYDAF LDAVCELVAE ADQRFGCKGS VGIGIPGMPE TEDGTLYAAN VPAASGKPLR ADLSARLDRD VRLDNDANCF ALSEAWDDEF TQYPLVMGLI LGTGVGGGLI FNGKPITGKS YITGEFGHMR LPVDALTMMG LDFPLRRCGC GQHGCIENYL SGRGFAWLYQ HYYHQPLQAP EIIALYDQGD EQARAHVERY LDLLAVCLGN ILTIVDPDLV VIGGGLSNFP AITTQLADRL PRHLLPVARV PRIERARHGD AGGMRGAAFL HLTD Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.