Description: Recombinant Human MMP9 Protein is produced by mammalian expression system and the target gene encoding Ala19-Asp707 is expressed with a 6His tag at the C-terminus. Form: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 2mM CaCl2, 150mM NaCl, 0.05% Brij35, pH7.5. Gene Symbol: MMP9 Alternative Names: CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELBEntrez Gene (Human): 4318SwissProt (Human): P14780Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : AAPRQRQSTL VLFPGDLRTN LTDRQLAEEY LYRYGYTRVA EMRGESKSLG PALLLLQKQL SLPETGELDS ATLKAMRTPR CGVPDLGRFQ TFEGDLKWHH HNITYWIQNY SEDLPRAVID DAFARAFALW SAVTPLTFTR VYSRDADIVI QFGVAEHGDG YPFDGKDGLL AHAFPPGPGI QGDAHFDDDE LWSLGKGVVV PTRFGNADGA ACHFPFIFEG RSYSACTTDG RSDGLPWCST TANYDTDDRF GFCPSERLYT RDGNADGKPC QFPFIFQGQS YSACTTDGRS DGYRWCATTA NYDRDKLFGF CPTRADSTVM GGNSAGELCV FPFTFLGKEY STCTSEGRGD GRLWCATTSN FDSDKKWGFC PDQGYSLFLV AAHEFGHALG LDHSSVPEAL MYPMYRFTEG PPLHKDDVNG IRHLYGPRPE PEPRPPTTTT PQPTAPPTVC PTGPPTVHPS ERPTAGPTGP PSAGPTGPPT AGPSTATTVP LSPVDDACNV NIFDAIAEIG NQLYLFKDGK YWRFSEGRGS RPQGPFLIAD KWPALPRKLD SVFEEPLSKK LFFFSGRQVW VYTGASVLGP RRLDKLGLGA DVAQVTGALR SGRGKMLLFS GRRLWRFDVK AQMVDPRSAS EVDRMFPGVP LDTHDVFQYR EKAYFCQDRF YWRVSSRSEL NQVDQVGYVT YDILQCPEDV DHHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.