Description: Recombinant Human MMP12 Protein is produced by Mammalian expression system and the target gene encoding Gly106-Cys470 is expressed with a 6His tag at the C-terminus. Form: Supplied as a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, 0.05% Brij35, pH 5.5. Gene Symbol: MMP12 Alternative Names: HME; Macrophage metalloelastase; MME; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12Entrez Gene (Human): 4321SwissProt (Human): P39900Purity : Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure : LPLNSSTSLE KNNVLFGERY LEKFYGLEIN KLPVTKMKYS GNLMKEKIQE MQHFLGLKVT GQLDTSTLEM MHAPRCGVPD VHHFREMPGG PVWRKHYITY RINNYTPDMN REDVDYAIRK AFQVWSNVTP LKFSKINTGM ADILVVFARG AHGDFHAFDG KGGILAHAFG PGSGIGGDAH FDEDEFWTTH SGGTNLFLTA VHEIGHSLGL GHSSDPKAVM FPTYKYVDIN TFRLSADDIR GIQSLYGDPK ENQRLPNPDN SEPALCDPNL SFDAVTTVGN KIFFFKDRFF WLKVSERPKT SVNLISSLWP TLPSGIEAAY EIEARNQVFL FKDDKYWLIS NLRPEPNYPK SIHSFGFPNF VKKIDAAVFN PRFYRTYFFV DNQYWRYDER RQMMDPGYPK LITKNFQGIG PKIDAVFYSK NKYYYFFQGS NQFEYDFLLQ RITKTLKSNS WFGCVDHHHH HH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.