Description: Recombinant E. coli LON Protein is produced by E.coli expression system and the target gene encoding Asn2-Lys784 is expressed with a 6His tag at the C-terminus. Form: Supplied as a 0.2 μM filtered solution of 50mM Tris-HCl, 100mM KCl, 10% Glycerol, pH 7.5. Gene Symbol: LON Alternative Names: capR; deg; lopA; muc; Lon protease; ATP-dependent protease LaEntrez Gene (Human): 945085 (E. coli)SwissProt (Human): P0A9M0 (E. coli)Purity : Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure : MGNPERSERI EIPVLPLRDV VVYPHMVIPL FVGREKSIRC LEAAMDHDKK IMLVAQKEAS TDEPGVNDLF TVGTVASILQ MLKLPDGTVK VLVEGLQRAR ISALSDNGEH FSAKAEYLES PTIDEREQEV LVRTAISQFE GYIKLNKKIP PEVLTSLNSI DDPARLADTI AAHMPLKLAD KQSVLEMSDV NERLEYLMAM MESEIDLLQV EKRIRNRVKK QMEKSQREYY LNEQMKAIQK ELGEMDDAPD ENEALKRKID AAKMPKEAKE KAEAELQKLK MMSPMSAEAT VVRGYIDWMV QVPWNARSKV KKDLRQAQEI LDTDHYGLER VKDRILEYLA VQSRVNKIKG PILCLVGPPG VGKTSLGQSI AKATGRKYVR MALGGVRDEA EIRGHRRTYI GSMPGKLIQK MAKVGVKNPL FLLDEIDKMS SDMRGDPASA LLEVLDPEQN VAFSDHYLEV DYDLSDVMFV ATSNSMNIPA PLLDRMEVIR LSGYTEDEKL NIAKRHLLPK QIERNALKKG ELTVDDSAII GIIRYYTREA GVRGLEREIS KLCRKAVKQL LLDKSLKHIE INGDNLHDYL GVQRFDYGRA DNENRVGQVT GLAWTEVGGD LLTIETACVP GKGKLTYTGS LGEVMQESIQ AALTVVRARA EKLGINPDFY EKRDIHVHVP EGATPKDGPS AGIAMCTALV SCLTGNPVRA DVAMTGEITL RGQVLPIGGL KEKLLAAHRG GIKTVLIPFE NKRDLEEIPD NVIADLDIHP VKRIEEVLTL ALQNEPSGMQ VVTAKLEHHH HHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.