Description: Recombinant Human HPRT1 Protein is produced by E. coli expression system and the target gene encoding Met1-Ala218 is expressed with a 6His tag at the N-terminus. Form: Liquid in a 0.2 μM filtered solution of 20mM Tris, 250mM NaCl, 2mM EDTA, 30% Glycerol, pH 8.0. Gene Symbol: HPRT1 Alternative Names: HPRT; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTaseEntrez Gene (Human): 3251SwissProt (Human): P00492Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKAVE HHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.