Description: Recombinant Human HMGB1 Protein is produced by E. coli expression system and the target gene encoding Pro92-Val176 is expressed with a MARI tag at the N-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 50mM HEPES, 500mM NaCl, 0.6mM DTT, pH 7.9. Gene Symbol: HMGB1 Alternative Names: HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1Entrez Gene (Human): 3146SwissProt (Human): P09429Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MARIDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAKG KPDAAKKGVV Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.