Description: Recombinant Human GCA Protein is produced by E.coli expression system and the target gene encoding Met1-Ile217 is expressed with a GST tag at the N-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.5. Gene Symbol: GCA Alternative Names: GCL; GrancalcinEntrez Gene (Human): 25801SwissProt (Human): P28676Purity : Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Chemical Structure : MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMAYP GYGGGFGNFS IQVPGMQMGQ PVPETGPAIL LDGYSGPAYS DTYSSAGDSV YTYFSAVAGQ DGEVDAEELQ RCLTQSGING TYSPFSLETC RIMIAMLDRD HTGKMGFNAF KELWAALNAW KENFMTVDQD GSGTVEHHEL RQAIGLMGYR LSPQTLTTIV KRYSKNGRIF FDDYVACCVK LRALTDFFRK RDHLQQGSAN FIYDDFLQGT MAI Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.