Description: Recombinant Human FN1 Protein is produced by E. coli expression system and the target gene encoding Pro1270-Ser1546 and Ala1721-Thr2016 is expressed. Form: Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. Gene Symbol: FN1 Alternative Names: FN; Fibronectin; FN; Cold-insoluble globulin; CIGEntrez Gene (Human): 2335SwissProt (Human): P02751Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MPTDLRFTNI GPDTMRVTWA PPPSIDLTNF LVRYSPVKNE EDVAELSISP SDNAVVLTNL LPGTEYVVSV SSVYEQHEST PLRGRQKTGL DSPTGIDFSD ITANSFTVHW IAPRATITGY RIRHHPEHFS GRPREDRVPH SRNSITLTNL TPGTEYVVSI VALNGREESP LLIGQQSTVS DVPRDLEVVA ATPTSLLISW DAPAVTVRYY RITYGETGGN SPVQEFTVPG SKSTATISGL KPGVDYTITV YAVTGRGDSP ASSKPISINY RTEIDKPSMA IPAPTDLKFT QVTPTSLSAQ WTPPNVQLTG YRVRVTPKEK TGPMKEINLA PDSSSVVVSG LMVATKYEVS VYALKDTLTS RPAQGVVTTL ENVSPPRRAR VTDATETTIT ISWRTKTETI TGFQVDAVPA NGQTPIQRTI KPDVRSYTIT GLQPGTDYKI YLYTLNDNAR SSPVVIDAST AIDAPSNLRF LATTPNSLLV SWQPPRARIT GYIIKYEKPG SPPREVVPRP RPGVTEATIT GLEPGTEYTI YVIALKNNQK SEPLIGRKKT DELPQLVTLP HPNLHGPEIL DVPST Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.