Description: Recombinant Human CLGN Protein is produced by mammalian expression system and the target gene encoding Glu20-Trp471 is expressed with a 6His tag at the C-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Gene Symbol: CLGN Alternative Names: CalmeginEntrez Gene (Human): 1047SwissProt (Human): O14967Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : EFMDDDVETE DFEENSEEID VNESELSSEI KYKTPQPIGE VYFAETFDSG RLAGWVLSKA KKDDMDEEIS IYDGRWEIEE LKENQVPGDR GLVLKSRAKH HAISAVLAKP FIFADKPLIV QYEVNFQDGI DCGGAYIKLL ADTDDLILEN FYDKTSYIIM FGPDKCGEDY KLHFIFRHKH PKTGVFEEKH AKPPDVDLKK FFTDRKTHLY TLVMNPDDTF EVLVDQTVVN KGSLLEDVVP PIKPPKEIED PNDKKPEEWD ERAKIPDPSA VKPEDWDESE PAQIEDSSVV KPAGWLDDEP KFIPDPNAEK PDDWNEDTDG EWEAPQILNP ACRIGCGEWK PPMIDNPKYK GVWRPPLVDN PNYQGIWSPR KIPNPDYFED DHPFLLTSFS ALGLELWSMT SDIYFDNFII CSEKEVADHW AADGWRWKIM IANANKPGVL KQLMAAAEGH PWVDHHHHHH Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.