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Recombinant Human CDKN1A Protein CRP1275
Product NameRecombinant Human CDKN1A Protein
Cat No: CRP1275
Source: E. coli
Reactivity: Human
Applications: E, WB, SDS-PAGE, MS
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
10 μg
$200
50 μg
$600
500 μg
$2400
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Description: Recombinant Human CDKN1A Protein is produced by E. coli expression system and the target gene encoding Ser2-Pro164 is expressed with a 6His tag at the C-terminus.
Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Gene Symbol: CDKN1A
Alternative Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
Entrez Gene (Human): 1026
SwissProt (Human): P38936
Purity : Greater than 95% as determined by reducing SDS-PAGE.
Chemical Structure : SEPAGDVRQN PCGSKACRRL FGPVDSEQLR RDCDALMAGC IQEARERWNF DFVTETPLEG DFAWERVRGL GLPKLYLPTG PRRGRDELGG GRRPGTSPAL LQGTAEEDHV DLSLSCTLVP RSGEQAEGSP GGPGDSQGRK RRQTSMTDFY HSKRRLIFSK RKPLEHHHHH H
Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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