Description: Recombinant Human CDK4 Protein is produced by E. coli expression system and the target gene encoding Met1-Glu303 is expressed with a 6His, T7 tag at the N-terminus. Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Gene Symbol: CDK4 Alternative Names: Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3Entrez Gene (Human): 1019SwissProt (Human): P11802Purity : Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure : MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMATSRY EPVAEIGVGA YGTVYKARDP HSGHFVALKS VRVPNGGGGG GGLPISTVRE VALLRRLEAF EHPNVVRLMD VCATSRTDRE IKVTLVFEHV DQDLRTYLDK APPPGLPAET IKDLMRQFLR GLDFLHANCI VHRDLKPENI LVTSGGTVKL ADFGLARIYS YQMALTPVVV TLWYRAPEVL LQSTYATPVD MWSVGCIFAE MFRRKPLFCG NSEADQLGKI FDLIGLPPED DWPRDVSLPR GAFPPRGPRP VQSVVPEMEE SGAQLLLEML TFNPHKRISA FRALQHSYLH KDEGNPE Directions for Use : Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control : Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability : Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.