Description: Rabbit polyclonal antibody to 5-HT2B Immunogen: Recombinant protein corresponding to human 5-HT2B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: HTR2B Alternative Names: 5-hydroxytryptamine receptor 2B; 5-HT-2B; 5-HT2B; Serotonin receptor 2BEntrez Gene (Human): 3357Entrez Gene (Mouse) : 15559Entrez Gene (Rat) : 29581SwissProt (Human): P41595SwissProt (Mouse) : Q02152SwissProt (Rat) : P30994Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of 5-HT2B expression in SW480 (A) whole cell lysates.
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Immunofluorescent analysis of 5-HT2B staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.