Description: Rabbit polyclonal antibody to VEGFA Immunogen: Recombinant protein corresponding to human VEGFA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: VEGFA Alternative Names: VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPFEntrez Gene (Human): 7422Entrez Gene (Mouse) : 22339Entrez Gene (Rat) : 83785SwissProt (Human): P15692SwissProt (Mouse) : Q00731SwissProt (Rat) : P16612Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of VEGFA expression in HEK293T (A), A549 (B), mouse liver (C) whole cell lysates.
-
Immunofluorescent analysis of VEGFA staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.