Description: Rabbit polyclonal antibody to CCR3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CCR3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CCR3 Alternative Names: CMKBR3; C-C chemokine receptor type 3; C-C CKR-3; CC-CKR-3; CCR-3; CCR3; CKR3; Eosinophil eotaxin receptor; CD193Entrez Gene (Human): 1232Entrez Gene (Mouse) : 12771Entrez Gene (Rat) : 117027SwissProt (Human): P51677SwissProt (Mouse) : P51678SwissProt (Rat) : O54814Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CCR3 expression in HepG2 (A), mouse liver (B), rat spleen (C) whole cell lysates.
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Immunohistochemical analysis of CCR3 staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.