Description: Rabbit polyclonal antibody to EPO Receptor Immunogen: Recombinant protein corresponding to human EPO Receptor. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: EPOR Alternative Names: Erythropoietin receptor; EPO-REntrez Gene (Human): 2057Entrez Gene (Mouse) : 13857SwissProt (Human): P19235SwissProt (Mouse) : P14753SwissProt (Rat) : Q07303Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EPO Receptor expression in K562 (A), mouse kidney (B), rat liver (C) whole cell lysates.
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Immunofluorescent analysis of EPO Receptor staining in mouse fetal cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.