Description: Rabbit polyclonal antibody to SIRT3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SIRT3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/50 - 1/100) Gene Symbol: SIRT3 Alternative Names: SIR2L3; NAD-dependent protein deacetylase sirtuin-3 mitochondrial; hSIRT3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3Entrez Gene (Human): 23410Entrez Gene (Mouse) : 64384SwissProt (Human): Q9NTG7SwissProt (Mouse) : Q8R104Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SIRT3 expression in 22RV1 (A), SW620 (B), MCF7 (C) whole cell lysates.
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  Immunohistochemical analysis of SIRT3 staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.