Description: Rabbit polyclonal antibody to NEFM Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human of human NEFM. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: NEFM Alternative Names: NEF3; NFM; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M proteinEntrez Gene (Human): 4741Entrez Gene (Mouse) : 18040Entrez Gene (Rat) : 24588SwissProt (Human): P07197SwissProt (Mouse) : P08553SwissProt (Rat) : P12839Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NEFM expression in SHSY5Y (A) whole cell lysates.
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Immunofluorescent analysis of NEFM staining in mouse brain. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.