Description: Rabbit polyclonal antibody to IREB2 Immunogen: Recombinant protein corresponding to human IREB2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: IREB2 Alternative Names: Iron-responsive element-binding protein 2; IRE-BP 2; Iron regulatory protein 2; IRP2Entrez Gene (Human): 3658Entrez Gene (Mouse) : 64602Entrez Gene (Rat) : 64831SwissProt (Human): P48200SwissProt (Mouse) : Q811J3SwissProt (Rat) : Q62751Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IREB2 expression in HEK293T (A), mouse kidney (B), rat liver (C) whole cell lysates.
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Immunofluorescent analysis of IREB2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).