Description: Rabbit polyclonal antibody to IRF3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human IRF3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: IRF3 Alternative Names: Interferon regulatory factor 3; IRF-3Entrez Gene (Human): 3661Entrez Gene (Mouse) : 54131SwissProt (Human): Q14653SwissProt (Mouse) : P70671Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IRF3 expression in Hela (A), NIH3T3 (B) whole cell lysates.
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  Immunohistochemical analysis of IRF3 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of IRF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated secondary antibody (red) in PBS at room temperature in the dark.