Description: Rabbit polyclonal antibody to IL-10 Immunogen: Recombinant fusion protein of human IL-10. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: IL10 Alternative Names: Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIFEntrez Gene (Human): 3586Entrez Gene (Mouse) : 16153Entrez Gene (Rat) : 25325SwissProt (Human): P22301SwissProt (Mouse) : P18893SwissProt (Rat) : P29456Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IL-10 expression in Jurkat (A) whole cell lysates.
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  Immunohistochemical analysis of IL-10 staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.