Description: Rabbit polyclonal antibody to SMAD5 Immunogen: Recombinant fusion protein of human SMAD5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SMAD5 Alternative Names: MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5Entrez Gene (Human): 4090Entrez Gene (Mouse) : 17129Entrez Gene (Rat) : 59328SwissProt (Human): Q99717SwissProt (Mouse) : P97454SwissProt (Rat) : Q9R1V3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SMAD5 expression in H460 (A) whole cell lysates.
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  Immunohistochemical analysis of SMAD5 staining in human colon  formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.