Description: Rabbit polyclonal antibody to DNMT3B Immunogen: Recombinant fusion protein of human DNMT3B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: DNMT3B Alternative Names: DNA (cytosine-5)-methyltransferase 3B; Dnmt3b; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; M.HsaIIIBEntrez Gene (Human): 1789Entrez Gene (Mouse) : 13436SwissProt (Human): Q9UBC3SwissProt (Mouse) : O88509Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DNMT3B expression in A549 (A), mouse testis (B), C6 (C) whole cell lysates.
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  Immunohistochemical analysis of DNMT3B staining in mouse liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.