Description: Rabbit polyclonal antibody to ANO1 Immunogen: Recombinant fusion protein of human ANO1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: ANO1 Alternative Names: DOG1; ORAOV2; TAOS2; TMEM16A; Anoctamin-1; Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2Entrez Gene (Human): 55107Entrez Gene (Mouse) : 101772SwissProt (Human): Q5XXA6SwissProt (Mouse) : Q8BHY3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ANO1 expression in LO2 (A), HeLa (B), mouse lung (C), rat skeletal muscle (D) whole cell lysates.
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Immunofluorescent analysis of ANO1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.