Description: Rabbit polyclonal antibody to ZNRF3 Immunogen: KLH-conjugated synthetic peptide of human ZNRF3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ZNRF3 Alternative Names: KIAA1133; RNF203; E3 ubiquitin-protein ligase ZNRF3; RING finger protein 203; Zinc/RING finger protein 3Entrez Gene (Human): 84133Entrez Gene (Mouse) : 407821SwissProt (Human): Q9ULT6SwissProt (Mouse) : Q5SSZ7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ZNRF3 expression in HT29 (A), mouse testis (B) whole cell lysates.
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Immunohistochemical analysis of ZNRF3 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.