Description: Rabbit polyclonal antibody to MIZ-1 Immunogen: Recombinant fusion protein of human MIZ-1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ZBTB17 Alternative Names: MIZ1; ZNF151; ZNF60; Zinc finger and BTB domain-containing protein 17; Myc-interacting zinc finger protein 1; Miz-1; Zinc finger protein 151; Zinc finger protein 60Entrez Gene (Human): 7709Entrez Gene (Mouse) : 22642SwissProt (Human): Q13105SwissProt (Mouse) : Q60821Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MIZ-1 expression in U87MG (A), HeLa (B) whole cell lysates.
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Immunohistochemical analysis of MIZ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.