Description: Rabbit polyclonal antibody to YTHDF2 Immunogen: Recombinant fusion protein of human YTHDF2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: YTHDF2 Alternative Names: HGRG8; YTH domain-containing family protein 2; CLL-associated antigen KW-14; High-glucose-regulated protein 8; Renal carcinoma antigen NY-REN-2Entrez Gene (Human): 51441Entrez Gene (Mouse) : 213541SwissProt (Human): Q9Y5A9SwissProt (Mouse) : Q91YT7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of YTHDF2 expression in Jurkat (A), mouse testis (B) whole cell lysates.
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Immunohistochemical analysis of YTHDF2 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.