Description: Rabbit polyclonal antibody to VPS25 Immunogen: Recombinant fusion protein of human VPS25. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: VPS25 Alternative Names: DERP9; EAP20; Vacuolar protein-sorting-associated protein 25; hVps25; Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25Entrez Gene (Human): 84313Entrez Gene (Mouse) : 28084Entrez Gene (Rat) : 681059SwissProt (Human): Q9BRG1SwissProt (Mouse) : Q9CQ80SwissProt (Rat) : P0C0A1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of VPS25 expression in BxPC3 (A), mouse brain (B), rat lung (C) whole cell lysates.
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  Immunofluorescent analysis of VPS25 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.