Description: Rabbit polyclonal antibody to VAMP-1 Immunogen: Recombinant fusion protein of human VAMP-1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: VAMP1 Alternative Names: SYB1; Vesicle-associated membrane protein 1; VAMP-1; Synaptobrevin-1Entrez Gene (Human): 6843Entrez Gene (Mouse) : 22317Entrez Gene (Rat) : 25624SwissProt (Human): P23763SwissProt (Mouse) : Q62442SwissProt (Rat) : Q63666Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of VAMP-1 expression in mouse brain (A), rat brain (B) whole cell lysates.
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Immunohistochemical analysis of VAMP-1 staining in rat spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.