Description: Rabbit polyclonal antibody to UBA3 Immunogen: Recombinant fusion protein of human UBA3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: UBA3 Alternative Names: UBE1C; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3Entrez Gene (Human): 9039Entrez Gene (Mouse) : 22200Entrez Gene (Rat) : 117553SwissProt (Human): Q8TBC4SwissProt (Mouse) : Q8C878SwissProt (Rat) : Q99MI7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of UBA3 expression in HepG2 (A), Jurkat (B), mouse heart (C), rat brain (D) whole cell lysates.
• 
  Immunohistochemical analysis of UBA3 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.