Description: Rabbit polyclonal antibody to TRIM63 Immunogen: KLH-conjugated synthetic peptide of human TRIM63. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: TRIM63 Alternative Names: IRF; MURF1; RNF28; SMRZ; E3 ubiquitin-protein ligase TRIM63; Iris RING finger protein; Muscle-specific RING finger protein 1; MuRF-1; MuRF1; RING finger protein 28; Striated muscle RING zinc finger protein; Tripartite motif-containing protein 63Entrez Gene (Human): 84676Entrez Gene (Rat) : 140939SwissProt (Human): Q969Q1SwissProt (Mouse) : Q38HM4SwissProt (Rat) : Q91Z63Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TRIM63 expression in mouse heart (A) whole cell lysates.
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Immunofluorescent analysis of TRIM63 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.